Bioinformatics Concepts

ChIP-seq and Peak Calling

ChIP-seq (Chromatin Immunoprecipitation Sequencing) is a method for identifying DNA regions bound by specific proteins, such as transcription factors. Antibodies are used to isolate protein-bound DNA fragments, which are then sequenced. The result is a genome-wide "peak map" that shows where proteins were most likely bound.

In this simulation, we use a synthetic genome of length 500 bases. 100 reads are generated based on how closely regions of the genome match a target binding site. You can adjust antibody specificity to simulate better or worse binding precision.