Sanger sequencing uses chain-terminating nucleotides (ddNTPs) to generate DNA fragments of varying lengths. The ratio of ddNTPs to dNTPs affects how often synthesis stops, influencing the distribution of fragment sizes.
This simulation models Sanger sequencing for a DNA sequence of length 100, allowing users to experiment with different ddNTP/dNTP ratios and observe their effects on fragment lengths. (Currently the simulation uses a theoretical N base. In the future, we will break up the simulation into four separate bases.)