In Illumina sequencing, phasing errors occur when not all DNA strands in a cluster incorporate a base at the same cycle. Over time, this leads to signal degradation, causing growing uncertainty in base calls as the read progresses.
This simulation shows how phasing affects the accuracy of base calling in a single read. As the read length increases, phasing errors accumulate, leading to more frequent mismatches. Investigate how error rate affects the accuracy of the reads.