Coverage in sequencing refers to the number of times a nucleotide position in the reference genome is read during sequencing. Higher coverage increases confidence in base calls and reduces sequencing errors, while lower coverage may result in gaps or uncertain regions. This tool allows users to visualize coverage by selecting read length and the number of reads to see how sequencing depth affects alignment.
Coverage is calculated using the formula:
\[
\text{Coverage} = \frac{L \times N}{G}
\]
where: